Face-down: Gravity's effects on cell movement

May 13, 2023 01:00 JST

Source: Science and Technology of Advanced Materials

Specially coated surfaces help scientists investigate what happens when cell clusters are turned upside down.

TSUKUBA, Japan, May 13, 2023 - (ACN Newswire) - Researchers at the National Institute for Materials Science (NIMS) and colleagues in Japan have developed a specially coated, light-responsive surface that helps test how the direction of gravity impacts cell movements. The findings, published in the journal Science and Technology of Advanced Materials, could lead to a better understanding of what happens to cells in people who are bedridden for prolonged periods and of the impact of gravity's direction on cancer cell migration.

The human body takes many different positions over its lifespan. Scientists wanted to find a way
to study how cell movement is impacted when the direction of gravity changes as our body positions
change. (Image created using materials from STAM Vol.24, Issue 1, Article 2206525 (2023) and
Canva.)

The special surfaces are made by coating glass slides with a combination of molecules that are responsive to light. Shining light on a central, circular area of the slide breaks up the molecules, clearing away a coating-free zone that cells can stick to. Once stabilized in this area, the scientists then use light to clear away an area surrounding the central circle. This encourages the cells to move in an outward direction to fill the square. The team investigated what happens to cell movement when the slide is placed upright, with the cells lying on top and the direction of gravity impacting the cells from top to bottom. They then conducted a similar test with the slide flipped over while supported on either side so that the cells are inverted and the direction of gravity is from the bottom of the cells to their tops.

"We found that the direction of gravity hindered collective cell migration in the inverted position by reducing the number of outward-moving leader cells at cluster edges and by redistributing shape-forming filaments, composed of actin and myosin, so that they kept the cells bundled together," explains biomaterials researcher, Shimaa Abdellatef, who is a postdoc at NIMS.

The coated, light-responsive surfaces provide an advantage over currently available methods that study the impacts of gravity's direction, as they require physical contact with the surface to which cells are attached. The new approach enables remote induction of cell migration.

"We plan to apply our approach to analyse the responses of cancer cells to the direction of gravity," says NIMS nanoscientist, Jun Nakanishi, who led the study. "We expect to find differences between healthy and diseased cells, which could provide important information about cancer progression in bedridden patients."

Further information
Jun Nakanishi
National Institute for Materials Science (NIMS)
Email:NAKANISHI.Jun@nims.go.jp

Shimaa A. Abdellatef
National Institute for Materials Science (NIMS)
Email: ABDELALEEM.shimaa@nims.go.jp

Paper: https://doi.org/10.1080/14686996.2023.2206525

About Science and Technology of Advanced Materials (STAM)

Open access journal STAM publishes outstanding research articles across all aspects of materials science, including functional and structural materials, theoretical analyses, and properties of materials. https://www.tandfonline.com/STAM

Dr Yasufumi Nakamichi
STAM Publishing Director
Email: NAKAMICHI.Yasufumi@nims.go.jp

Press release distributed by Asia Research News for Science and Technology of Advanced Materials.

Source: Science and Technology of Advanced Materials
Sectors: Materials & Nanotech, Science & Research, BioTech, Healthcare & Pharm